#/usr/bin/perl -w
use strict; my ($len,$total)=(0,0); my @x; while(<>){ if(/^\>\@/){ if(len>0) total+=$len; push @x,$len; } len=0; else s///g; len+=length(); if (len>0){ $total+=$len; push @x,len; @x=sortb<=>a @x; my (count,$half)=(0,0); for (my $j=0;$j<@x;$j++){ $count+=$xj; if (($count>=$total/2)&&(half==0)) print "N50:xj\n"; $half=$xj }elsif ($count>=$total*0.9){ print "N90: $x$j\n"; exit; }
N50 N90 calculation using Perl code
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